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Three people died and seven were seriously injured in a head-on collision between a mini-bus taxi and a truck on the road between Mthatha and Mqanduli on Wednesday afternoon.
The Department of Transport said all the victims were in the mini-bus.
"The truck driver escaped unharmed," said spokesperson, Unathi Binqose.
He added that the injured passengers were rushed to a nearby hospital.
"A culpable homicide docket has been opened," Binqose said.