Christopher Panayiotou, accused of master-minding the murder of his wife Jayde, had an on and off relationship with his employee Chanelle Coutts.
This was according to his lawyer Alwyn Griebenow on Wednesday, Netwerk24 reported.
On speculation that Coutts could be pregnant, Griebenow said: "If she is pregnant Christopher is not aware of it."
Coutts's lawyer, Danie Gouws, dismissed the rumours of pregnancy and tests as "absurd".
Coutts, the personnel manager at the OK Grocer in Algoa Park, was not at the Port Elizabeth Magistrate's Court where Panayiotou was applying for bail.
Panayiotou, 28, Thando Siyoni, 31 and Sizwezakhe Vumazonke, 30, were arrested in the days following Jayde's disappearance.
She was last seen waiting outside their home in Kabega Park for her lift to Riebeeck Park High School on Tuesday, April 21. She was found dead outside Kwanobuhle township in Uitenhage the following day.
Panayiotou is facing charges of conspiracy to commit murder; robbery with aggravated circumstances; kidnapping; murder; and defeating the ends of justice.
Magistrate Abigail Beaton postponed the matter to Thursday.
