Eastern Cape Education Superintendent-General Modidima Mannya says striking teachers are violating the rights of children to education, adding that there will be consequences.
Mannya has also reminded school principals of their responsibilities in the event of any form of industrial action.
Teachers in at least five centres in the province are protesting against long hours and a heavy workload caused by the dismissal of temporary teachers.
Provincial education department spokesperson Loyiso Pulumani says schools in Port Elizabeth, Uitenhage, Queenstown, Matatiele and Mthatha are known to be have been affected.
However, he says it could be more widespread.
Meanwhile, Bhisho's Portfolio Committee on Education concludes a programme of school visits today.
The aim of the visits is for members to get first-hand insight into matters such as the availability of learner support material, state of infrastructure and the provision of transport and nutrition.
The committee visited eight schools in the Nelson Mandela Metro, the Cacadu District, Mthatha, Kokstad, Queenstown, Amathole and Aliwal North.
The Eastern Cape Provincial Legislature says the school visit programme is conducted annually at the beginning of the year to assess the state of readiness of schools.