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Mount Camdeboo Private Game Reserve in the Eastern Cape says its devastated by the loss of a second rhino to poachers so far this year.
Spokesperson, Richard Slater says in a statement the female rhino, known as "Split Horn", was poached for her horn over the weekend.
He says the animal had a young calf with her when she was darted by the poachers.
Slater says both horns were removed.
"Split horn" has been on the reserve for a number of years, gave birth to three animals and raised them successfully.
Slater says since the loss of a male rhino in May, they have taken every effort to increase security measures, including the appointment of a dedicated Anti-Poaching Unit.
