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Pukke handed defending champions UP-Tuks their first defeat of the 2014 FNB Varsity Cup on Monday night to claim top spot on the standings.
Coach Robert du Preez's team were ruthless in wet conditions in Pretoria as they shut out the home side to claim a convincing 18-0 victory and leapfrog them on the log in the process.
UCT recorded their third away win of the season in Johannesburg, scoring five tries on their way to a 37-29 victory over UJ to move up to third position.
The top four after five weeks of action is rounded off by Shimlas who ended deadlocked at 13-13 with three-time champions Maties in their clash in Stellenbosch.
The NMMU Madibaz were forced to work hard for their 18-12 victory over Wits in driving rain at the Wits campus in Johannesburg.
The Eastern Cape side scored on the stroke of fulltime to secure the victory after the game had hung in the balance in the final stages.
The Madibaz moved up to fifth spot and remain within a shout of a playoff spot this year.