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Two people were killed in a head-on collision on the R335 Addo road near Gqeberha on Saturday evening.
The Eastern Cape Transport Department says a Mazda Etude collided with an articulated truck.
Spokesperson, Unathi Binqose says both men in the Mazda died on impact, while the truck driver escaped unharmed.
He says the cause of the accident is still unknown.
Swartkops police are investigating a case of culpable homicide.