Four people died and three others were rushed to hospital following a head on collision on the R72 between East London and Port Alfred on Friday night.
Khuselwa Rantjie from the Department of Transport says a Toyota Quantum Dynamics Ambulance with two occupants was travelling from East London towards Port Alfred when it collided head on with a Suzuki sedan with five occupants coming from Port Alfred.
Its alleged that the ambulance lost control while avoiding cattle in the road and smashed head on into the Suzuki.
Four occupants of the sedan, including the driver, were declared dead on the scene while one passenger sustained critical injuries. The seriously injured ambulance driver was trapped in his vehicle while his assistant sustained only slight injuries.
Rantjie said the R72 is one of the deadly roads in the province with stray animals contributing to the problem.
The MEC for Transport in the Province has sent her condolences to the families of the deceased and appealed to livestock owners to take responsibility for their animals.
The Chalumna SAPS have open a case of culpable homicide.
