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The High Court in Pretoria will hear an application by the Opposition to Urban Tolling Alliance - Outa on Friday to appeal against a previous judgment approving e-tolling.
Outa said on Tuesday that it remained "committed to the case".
The appeal follows a judgment in December by the same court, which dismissed Outa's application to have the electronic tolling of Gauteng's major roads scrapped.
The court ordered Outa to pay the legal costs of the application.