The DA says the SA National Roads Agency Limited must disclose the names of all 33 sub-contractors involved in the collection of e-tolls on Gauteng highways.
Party spokesperson, Jack Bloom says the demand follows after disturbing reports today about ANC links to companies that will benefit from e-tolling.
The DA has asked Public Protector, Thuli Madonsela to investigate the controversial contracts.
The Sunday Times has reported that politically-connected companies stand to benefit from e-tolling contracts.
According to the newspaper, these include Tsebo Holdings, South Africa's largest catering company, which is 15 percent owned by Nozala Investments and 15 percent by Lereko.
Nozala is headed by Salu-kazi Dakile-Hlongwane, a trustee of the ANC front company Chancellor House, and Lereko is owned by former environmental minister, Valli Moosa and Chancellor House trustee Popo Molefe.