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The three leading women's seeds and men's number one Richard Gasquet were toppled in short order on Tuesday at the Eastbourne grass-court event with Wimbledon winner Petra Kvitova leading the exit.
The Czech, seeded second, was joined on the sidelines by top seed Agnieszka Radwanska and number three Caroline Wozniacki.
Kvitova lost 7-5, 6-4 to Ekaterina Makarova and Radwanska also lost in straight sets to Bulgarian Tsvetana Pironkova.
Wozniacki fell to American Christina McHale 6-1, 6-7 (7/9), 6-4.
Men's top seed Gasquet joined the day's upset list a week before the start of Wimbledon as the Frenchman lost to Australian Marinko Matosevic 1-6, 7-6 (7/5), 7-6
